賽爾瑞成(CRGEN)生產(chǎn)的重組nCoV S1蛋白由原核系統(tǒng)表達、經(jīng)分離純化和過濾除菌等步驟制成,分子量約為75.3 kDa。
氨基酸序列:
MSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFA STEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFL MDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAG AAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATR FASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDF TGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVV VLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGV S VITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQ TNSPRRARLEHHHHHH
表達宿主: E. coli
保存緩沖液
25 mM 硼酸,0.1%吐溫80,pH 9.2
保存條件
本產(chǎn)品經(jīng)無菌過濾后保存于-20℃冰箱,一般應(yīng)避免反復(fù)凍融。建議將蛋白分裝成小份,每次使用一份。
質(zhì)量檢測:
純度:經(jīng)SDS-PAGE電泳檢測純度>90%.
穩(wěn)定性:樣品在-20℃保存12個月穩(wěn)定
發(fā)表的文章:
1. Zhang Jian et al. Real-time, selective, and low-cost detection of trace level SARS-CoV-2 spike-protein for cold-chain food quarantine. npj Science of Food, 2021, 5(1): 12.
貨號 |
產(chǎn)品名稱 |
規(guī)格 |
目錄價格 |
PCS101 |
重組nCoV S1蛋白 |
100 μg |
1700 |
PCS102 |
重組nCoV S1蛋白 |
500 μg |
3780 |
PCS103 |
重組nCoV S1蛋白 |
1000 μg |
5250 |